UV755B UV756 UV759 V723N V7230G Spectrophotometers | UV-Vis

SINGLE-BEAM UV-VIS SPECTROPHOTOMETERS

UV755B UV756 UV759 V723N V7230G Spectrophotometers

The UV755B, UV756, UV759, V723N, and V7230G are single-beam spectrophotometers for absorbance, transmittance, energy, and quantitative analysis. Depending on the model, they cover ultraviolet and visible wavelengths or the visible range, with automatic wavelength setting and a 2 nm or 4 nm spectral bandwidth.

Use photometric mode to measure sample absorbance or transmittance. For quantitative analysis, build a standard curve from reference samples or enter known curve coefficients to calculate an unknown concentration. The instruments also store test data and standard curves, and can print data and curves.

Key Features

Optical System	Single-beam optical system with a 1,200 lines/mm grating
Photometric Modes	Measurement modes for absorbance, transmittance, concentration, and energy
Quantitative Analysis	Using a standard curve or the coefficient method
Automatic Functions	Automatic wavelength and filter calibration, automatic lamp switching, and startup self-checks
Data Storage	Storage for up to 4,000 test results and 128 standard curves
Replaceable Lamps	Replaceable socket-type deuterium and tungsten lamps on UV759, UV756, and UV755B models
Sample Chamber	Large sample chamber accepts cuvettes from 5 to 100 mm
Data Output	USB and RS-232 outputs

Five-Model Comparison

Model	Wavelength range	Spectral bandwidth	Wavelength accuracy	Stray light	Light source
UV759	190–1,100 nm	2 nm	±0.3 nm	0.05%T	Deuterium and tungsten lamps
UV756	190–1,100 nm	2 nm	±0.5 nm	0.1%T	Deuterium and tungsten lamps
UV755B	190–1,100 nm	2 nm	1 nm	0.15%T	Deuterium and tungsten lamps
V723N	320–1,100 nm	2 nm	±0.5 nm	0.05%T	Tungsten lamp
V7230G	320–1,100 nm	4 nm	1 nm	0.15%T	Tungsten lamp

Wavelength repeatability: ≤0.1 nm for UV759 and V723N; ≤0.2 nm for UV756, UV755B, and V7230G. Photometric accuracy: 0.3%T for UV759 and V723N; 0.5%T for UV756, UV755B, and V7230G. Stability at 500 nm: ±0.0001 A/h for UV759 and V723N; ±0.0002 A/h for UV756, UV755B, and V7230G.

Common Specifications

Parameter	Value
Optical system	Single beam; grating, 1,200 lines/mm
Photometric repeatability	0.2%T
Photometric range	0–200%T; −0.3 to 3 A; 0–9,999 C
Baseline flatness	±0.002 A
Noise	±0.001 A
Work modes	T, A, C, E
Wavelength setting	Automatic
Display	128 × 64 LCD
Detector	Silicon photodiode
Data output	USB, RS-232
Power	110 V or 220 V, as configured
Standard accessories	4 × 10 mm glass cuvettes; 2 × 10 mm quartz cuvettes (UV); power cord; user manual; UV PC software

Operation and Data Handling

At startup, the instrument checks the filter, lamp switch, detector, lamps, wavelength calibration, and dark calibration. A 20-minute warm-up is recommended; the manual also states that the warm-up can be skipped. Before measuring, perform zero calibration with the reference solution in the optical path. Dark-current calibration may be needed when ambient temperature, ambient light, or operating voltage changes.

For quantitative testing, create and save a standard curve from one to nine standards, select a stored curve, or enter the coefficient method parameters. The coefficient method calculates concentration using C = K × A + B. Use cuvettes with consistent optical quality when preparing a standard curve.

Applications

These spectrophotometers support routine photometric and concentration measurements. With the compatible PC software, the system can also be used for spectral scanning, kinetics, DNA and protein analysis, and multi-wavelength testing.

Optional

50 mm or 100 mm manual four-cell holder; automatic eight-cell holder; film holder; additional software described in the source as 21 CFR-compliant — confirm the software version and compliance scope for your intended use.

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